Italiano

Fondamenti delle principali tecniche analitiche impiegate nel laboratorio di biochimica clinica
Dosaggio e significato delle più comuni indagini di laboratorio di biochimica Clinica applicabili in gravidanza e non.

Biochimica Clinica e Medicina di Laboratorio. Marcello Ciaccio, Giuseppe Lippi. EdiSES

Acquisire i concetti fondamentali circa i metodi e tecniche di dosaggio e il significato clinico delle principali indagini di laboratorio di biochimica clinica

E' richiesta buona conoscenza delle materie di base: Chimica, Biologia, Biochimica. Gli studenti/le studentesse devono aver seguito i corsi d'obbligo e aver acquisito le nozioni propedeutiche. L'accesso all'esame è regolato dal superamento degli esami propedeutici, secondo quanto stabilito dal Consiglio di Corso di Laurea.

L'Insegnamento sarà svolto mediante lezioni frontali in Lingua Italiana con l'ausilio di slides o altro materiale informatico.

La verifica dell’apprendimento sarà effettuato mediante esame orale finale, con votazione espressa in trentesimi. Lo studente dovrà dimostrare capacità di discutere in maniera critica sugli argomenti trattati, nonché di collegamento delle conoscenze acquisite. La media aritmetica dei moduli che compongono il corso integrato determinerà invece il voto finale.

Inoltre agli studenti sarà fornito dal docente il materiale didattico utilizzato per le lezioni, o in forma cartacea o di PowerPoint. Tale materiale si intende ad esclusivo uso esplicativo e non sostituisce in alcun modo il testo.
Per qualsiasi altra problematica inerente gli argomenti del Corso gli studenti possono rivolgersi al docente di riferimento, secondo l'orario e le modalità di ricevimento forniti dal docente.

• Definizione e finalità della Biochimica Clinica. Generalità.
• Variabilità pre-analitica, analitica, post-analitica. Variabilità biologica. Intervallo di riferimento. Attendibilità, precisione, accuratezza, specificità e sensibilità analitica. Errori di misura. Controllo di qualità. Il referto di laboratorio
• Fondamenti delle principali tecniche analitiche impiegate nel laboratorio di biochimica clinica
• Elettroforesi delle proteine sieriche
• Esame emocromocitometrico
• Esame standard delle urine
• Enzimi in biochimica clinica
• Lipoproteine e assetto lipidico
• Dosaggio e significato delle seguenti indagini di laboratorio: uremia, creatininemia, bilirubinemia, uricemia, sideremia, transferrinemia, ferritinemia, calcemia, fosforemia, sodiemia, potassiemia. Proteina C Reattiva
• Adipochine, obesità e sindromi correlate

Italian

Methodology and Laboratory Diagnostics in Medicine.
This course describes the basic and fundamental knowledge on the determination methods and clinical significance of the most common tests of clinical biochemistry involved in Pregnancy e not only.

Reference Test:
Marcello Ciaccio, Clinical and Laboratory Medicine Textbook, Springer Nature, edition I/2024, pages 739.

Acquire the ability to orientate correctly in laboratory diagnostics
- Know the main laboratory diagnostic tests
- Knowing how to correctly interpret the results of laboratory tests
- Be able to correctly apply the concepts of laboratory medicine to diagnostics, differential diagnostics and evaluation of prognosis
- Apply semeiotics and laboratory diagnostics to clinical practice

Knowledges and skills furnished by the course of Biochemistry. More in details, to attend the course, the students will need to have a well-established background as specifically indicated by The Council of the TLB school respecting preparatory activities where it applies.

Frontal lessons
Articulated Frontal Lessons: Integrations between the various didactic modules of the integrated course
Single theme seminars
Theoretical-Practical Lessons
The course consists of lectures using slides and other multimedia tools. For same topics, defined at the beginning of the course together with the students, a theoretical and practical approach will be applied, including discussion of laboratory reports and simulation of problem-solving methods.

Oral exam aimed at highlighting:
a) learning the various course topics;
b) the candidate's ability to express himself/herself correctly in medical-technical language
c) ability of the candidate to orient himself/herself correctly in typical situations that he will encounter in medical practice;
d) ability of the candidate to choose the diagnostic profiles suitable for typical situations and correctly interpret the results for the purposes of general diagnostics, differential and prognostic evaluation. The final mark is expressed in 30/30, where 18/30 represents the minimum and 30/30 the maximum.

The assessment of the knowledge and skills will be carried out by oral exam aimed at highlighting: i) the clarity of exposition, completeness, and terminological precision; ii) the ability of choosing the diagnostic profiles suitable for typical situations; iii) the skills of interpretating the results for the purposes of general diagnostics, differential and prognostic evaluation. The grade of Clinical Biochemistry and Clinical Molecular Biology is expressed in 30/30, where 18/30 represents the minimum and 30/30 the maximum. The final mark of the exam is given by the mean among the different teaching modules that make up the integrated course of Laboratory Medicine (Clinical Pathology, Clinical Microbiology and Clinical Biochemistry and Clinical Molecular Biology), always expressed in 30/30.

The lecture slides as well as literature references relevant to the course topics will be provided to the students
The lecture slides, as well as all the relevant references from the scientific literature, will be shared by Microsoft Teams. Students can directly reach the designed Teacher for any clarification or further explanation as indicated at the page of the appointed Professor.

• Definition and purpose of Clinical Biochemistry. Generality.

• Pre-analytical, analytical, post-analytical variability. Biological variability. Reference range. Reliability, precision, accuracy, specificity and analytical sensitivity. Measurement errors. Quality control. The laboratory report
• Electrophoresis of serum proteins

• Haemochromocytometric examination

• Standard urine test
• Enzymes in clinical biochemistry
• Lipoproteins and lipid profile
• Dosage and significance of the following laboratory investigations: uremia, creatininemia, bilirubinemia, uricemia, sideremia, transferrinemia, ferritinemia, calcemia, phosphoremia, sodiemia, potassium. C-reactive protein
• Adipokine, obesity and related syndromes